Oly Blast 4 using refseq_genomic db

In [*]: 
!blastn -query /Volumes/web/scaphapoda/Hannah/Olurida_transcriptome_v3.fasta -db /Volumes/web/whale/blast/db/env_nt -out /Volumes/web/scaphapoda/Hannah/Blast/Oly_Blast_4_refseq_genomic.txt -outfmt 6 -evalue 1E-5 -max_target_seqs 1 -num_threads 4

Modified Oly Blast 4

In [2]: 
!tr '|' "\t" </Volumes/web/scaphapoda/Hannah/Blast/Oly_Blast_4_refseq_genomic.txt> /Volumes/web/scaphapoda/Hannah//Blast/Oly_Blast_refseq_genomic.txt
In [3]: 
!head /Volumes/web/scaphapoda/Hannah//Blast/Oly_Blast_4_mod.txt

comp2352_c0_seq1	gi	355578640	gb	AGTN01730703.1		90.79	239	21	1	5	242	130	368	2e-84	 318
comp7079_c0_seq1	gi	475683069	dbj	FX012446.1		79.06	191	40	0	557	747	296	106	2e-27	 132
comp8088_c0_seq1	gi	130374143	gb	AACY023318861.1		100.00	254	0	0	1	254	800	547	6e-130	 470
comp8243_c0_seq1	gi	130994115	gb	AACY022703208.1		81.74	115	19	2	1624	1737	607	720	4e-16	95.3
comp8371_c0_seq1	gi	152151037	gb	ABEF01053994.1		82.93	82	14	0	427	508	2165	2246	6e-10	75.0
comp9857_c0_seq1	gi	212490942	gb	AAUQ01003439.1		87.96	191	19	4	9	197	930	1118	2e-55	 222
comp10057_c0_seq1	gi	132564728	gb	AACY021147114.1		78.36	684	142	6	186	866	2	682	6e-120	 438
comp10057_c1_seq1	gi	494333443	gb	APMI01083662.1		88.74	604	66	2	1	603	651	49	0.0	 737
comp10066_c0_seq1	gi	494410819	gb	APMI01038473.1		80.51	236	46	0	139	374	11856	11621	1e-42	 182
comp10093_c0_seq1	gi	346602564	gb	JP004222.1		78.18	793	169	4	332	1122	763	1553	5e-139	 503

Oly Blast 8 using nr database

In []: 
!blastx -query /Volumes/web/scaphapoda/Hannah/Olurida_transcriptome_v3.fasta -db /Volumes/web/whale/blast/db/nr -out /Volumes/web/scaphapoda/Hannah/Blast/Oly_Blast_nr.txt -outfmt 6 -evalue 1E-5 -max_target_seqs 1 -num_threads 12
!tr '|' "\t" /Volumes/web/scaphapoda/Hannah/Blast/Oly_Blast_8_mod.txt

Oly Blast 13 using swissprot database

In [2]: 
!blastx -query /Volumes/web/scaphapoda/Hannah/Olurida_transcriptome_v3.fasta -db /Volumes/web/whale/blast/db/swissprot -out /Volumes/web/scaphapoda/Hannah/Blast/Oly_Blast_swissprot.txt -outfmt 6 -evalue 1E-5 -max_target_seqs 1 -num_threads 10

Error: NCBI C++ Exception:
    "/Users/coremake/release_build/build/PrepareRelease_IntelMAC_JSID_01_478_130.14.22.10_9052_1363182621/c++/src/corelib/ncbiobj.cpp", line 925: Critical: ncbi::CObject::ThrowNullPointerException() - Attempt to access NULL pointer.
In []: